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Showing 2 results for Aghaei Bahman Beglo

Amir Shapoori , Habib Asgharpour , Parvin Farzanegi , Neda Aghaei Bahman Beglou ,
Volume 18, Issue 4 (Jul-Aug 2024)
Abstract

Background: One of the causes of infertility is azoospermia. The present study aimed to investigate the effects of swimming exercise, cell therapy, and laser therapy on the expression of genes involved in mitochondrial dynamics in the testicular tissue of azoospermic rats.
Methods: In this experimental study, 40 rats (6-8-weeks old) were randomly assigned to 8 groups: 1) Healthy control, 2) patient, 3) sham, 4) laser, 5) exercise, 6) cell, 7) cell + laser, and 8) cell + exercise. The azoospermia model was induced using busulfan at a dose of 40 mg. Stem cells were transplanted once into the vas deferens at a concentration of 1000000 cells per rat. One week after cell transplantation, laser therapy was applied in three repetitions throughout the study period, with an interval of once a week, and after wound healing, the rats swam for 30 minutes a day, 5 days a week, for 8 weeks. To investigate the expression of the studied genes, tissue analysis was performed by the real-time polymerase chain reaction (RT-PCR) technique.
Results: Azoospermia significantly reduced the expression of mitofusin 2 (Mfn2) and significantly increased the expression of dynamin-related protein 1 (Drp1) and muscle-specific RING finger protein 1 (Murf1) in testicular tissue. The use of interventional methods increased the expression of Mfn2, and significantly reduced the expression of Murf1.
Conclusion: Based on the findings, the best changes were observed in the exercise + laser group. Therefore, it can be said that in rats using the azoospermia model, the simultaneous use of regular exercise interventions and laser therapy is the most effective.

 

Mahshad Paziraee , Habib Asgharpour , Asra Askari , Reza Rezaei Shirazi , Neda Aghaei Bahman Beglo ,
Volume 18, Issue 6 (Nov-Dec 2024)
Abstract

Background: Running and starvation can have a positive effect on the reticulophagy of the liver tissue. The purpose of this research was to investigate the effect of running and starvation interventions on the atherogenic index and Xbp1 gene change in the liver endoplasmic reticulum of non-alcoholic fatty liver rats (NAFLD).
Methods: Thirty obese male Wistar rats aged 18-20 weeks with an average body weight of 348 ± 25.53 grams, after one week of familiarization with the laboratory environment, were randomly divided into six groups (n=5 per group): 1) starvation, 2) three days of training, 3) five days of training, 4) three days of training plus starvation, 5) five days of training plus starvation, and 6) the control group. All fatty liver animal models had free access to water and standard food pellets (10 gr per 100 g of mouse body weight). The statistical test of one-way analysis of variance was used at a significance level of less than 0.05, and the LSD post-hoc test was used to compare research groups.
Results: According to the experimental results and statistical analyses (One-way analysis of variance), a significant decrease was noticed in the ratio of lipoproteins (VLDL/HDL and LDL/HDL) in all experimental groups compared to the control group. Also, a significant decrease was observed in the expression of XBP1 and CHOP genes in animals doing 3 and 5 days of exercise alone or along with starvation.
Conclusion: Regular exercise for 3 and 5 days per week with starvation can possibly reduce the activity of the genes involved in endoplasmic reticulum stress in NAFLD patients.

 


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