Showing 6 results for Davoodi
H Davoodi, S R Hashemi, H F Seow, M Ghorbani,
Volume 3, Issue 2 (Autumn – Winter 2010[PERSIAN] 2009)
Abstract
Abstract Background and objectives: Paraffin-embedded tissues and clinical samples are a valuable resource for molecular genetic studies, but the extraction of high-quality genomic DNA from this tissues is still a problematic issue. In the Present study, the efficiency of two DNA extraction protocols, a commercial kit and a traditional method based on heating and K Proteinase was compared. Material and Methods: Fifty paraffin-embedded blocks of colon cancer tissues (more than 5 years old) were used to compare two methods of DNA extraction. DNA was extracted by traditional method using heat and commercial DNA extraction (Qiagen kit) method. Then the purity and concentration of extracted DNA were measured by Spectrophotometer. Two sequences of TLR4 “The most important receptors in innate immunity” were amplified by polymerase chain reaction. SH-1 ‘188bp’ and SH-2 ‘124bp’ were amplified and then the products were separated on a 2% agarose gel. Results: The results show that the yield of DNA by traditional method (297 mg/ml) is significantly (p<0.01) higher than Commercial kit (176mg/ ml). But traditional method has the lower OD ratio (1.2) Compared to Commercial method. The Amplification of the TLR4 gene sequences is more successful by the traditional method (p<0.01) compared with commercial method. The length of the sequence affects on the results of PCR in that short sequence is amplified more successful compared to the long sequence. Conclusion: The traditional method is more successful in PCR amplification and also simple and cheap. Therefore, we recommend using this method for DNA extraction taken from the paraffin-embedded blocks with more than 5 years old and selecting shorter sequence for better amplification in PCR. Key words: DNA Extraction, paraffin embedded tissue, PCR
A Ebrahimzadeh, S Mohammadi, T Davoodi, Ar Salimi Khorashad, A Jamshidi,
Volume 7, Issue 3 (Autumn 2013)
Abstract
Abstract
Background and Objective: Toxoplasmosis is one of the most prevalent parasitic infections worldwide. Contamination of pregnant women with toxoplasmosis may cause fetal death, preterm delivery and congenital toxoplasmosis. Due to importance of congenital Toxoplasmosis and the need of further study, this research was accomplished in Zahedan.
Material and Methods: The serum samples (N= 221) were collected from pregnant women referring to reference laboratory of Zahedan in 2011. The IgG and IgM antibody levels against toxoplasmosis were investigated using ELISA method.
Results: Out of all samples, 30.8% are IgG positive and 1.4% are both IgG and IgM positive. There is no significant difference between positive and negative groups using Chi-square tests.
Conclusion: The main part of pregnant women in Zahedan (69.2%) is serologically negative against toxoplasmosis therefore, hygiene education to eliminate risk factors especially during pregnancy period seems to be imperative.
Keywords: ELISA Antibody Pregnancy Toxoplasma Zahedan
Pashaie Naghadeh, A, Dabirzadeh, M, Davoodi, T, Hashemi, M,
Volume 9, Issue 2 (may,jun 2015[PERSIAN] 2015)
Abstract
Background and objective: Bioindicators of drinking water are always influenced by physical and chemical factors such as turbidity and chlorine. Considering the assessment of drinking water quality is based on residual chlorine, E.coli, heterotrophic bacteria and turbidity. We aimed to evaluate the effect of pH, chlorine residual and turbidity on the microbial bioindicators.
Material and methods: In this descriptive-analytic study, 324 and 32 water samples were collected from rural and urban water distribution network of Aq Qala city in 2013, respectively. All steps were performed according to standard methods.
Results: In rural water supply, 5%, 9% and 33% of the samples were contaminated with fecal coliform, fecal streptococcus and the heterotrophic more than 500CFU / ml. In urban network, coliform contamination was not seen and other bioindicators were less than those of rural networks were. Turbidity of above 5 NTU in urban and rural samples was 3 and 9 percent, respectively. Bioindicators had significant relationship with residual chlorine, fecal coliform bacteria with pH and turbidity with heterotrophic bacteria (P ≤0.05).
Conclusion: The presence of fecal streptococcus bacteria in some samples without fecal coliform cannot confirm the safety of drinking water. Microbial contamination in the presence of residual chlorine implies that just chlorination is not enough for having healthy water.
Keywords: Chlorine, Turbidity, Biological Factors, Drinking water
Adel Ebrahimzadeh, Tahereh Davoodi , Abbas Pashaei Naghadeh ,
Volume 9, Issue 4 (sep,Oct 2015 2015)
Abstract
Abstract
Background and Objectives: Plasmodium falciparum merozoite surface protein-1 (PfMSP-1) is a promising vaccine against malaria during its blood stages which play an important role in immunity to this disease. Polymorphic nature of this gene is a major obstacle in making an effective vaccine against malaria. In this study, the genetic diversity of Plasmodium falciparum isolates was investigated in Sistan-Baluchestan Province using allelic families of the MSP-1.
Methods: From March/April 2011 to August/September 2012, 94 blood samples were collected from patients with falciparum malaria who were living in four districts of Sistan-Baluchestan Province. The extracted genomic DNA and genetic diversity of MSP-1 block 2 were evaluated by nested polymerase chain reaction.
Results: From a total of 94 patients, 89 patients (94.7%) had positive PCR results and the remaining five patients were excluded. Seven different alleles of MSP-1 were identified through size difference on agarose gel. Comprising 46.1% of the samples, MAD20 was identified as the predominant MSP-1 allelic family, while the RO33 family had the lowest frequency (with 7.9%). In 10% of samples infection with two alleles was observed.
Conclusion: The results of this study suggest that genetic diversity of PfMSP-1 in Southeastern Iran is relatively low and most infections originate from a clone that is consistent with an area of low malaria transmission. This information is useful for the prevention and control of malaria in Iran.
Keywords: Merozoite Surface Protein 1, Plasmodium Falciparum, Polymerase Chain Reaction
Mansour Dabirzadeh , Abbas Pashaie Neghadeh , Tahere Davoodi , Mohammad Hashemi ,
Volume 10, Issue 2 (Mar,Apr2016 2016)
Abstract
ABSTRACT
Background and Objective: Cutaneous leishmaniasis is a parasitic disease and a health problem in different parts of Iran, especially two cities of Mashhad and Chabahar. Due to morphological similarities of most Leishmania species and difference in reservoirs of L. major and L. tropica, it is necessary to determine the parasite specie to combat the disease. Thus, this study used gene sequencing and genotyping of 70-kDa heat shock protein (HSP70) to differentiate the two species of Leishmania.
Methods: In this descriptive-analytical study, microscope slides and cultures were prepared from 43 patients suspected of cutaneous leishmaniasis in Chabahar and Mashhad. PCR was performed after genomic DNA extraction and then PCR products were sequenced and analyzed.
Results: Of the 43 patients studied, 32 direct smear and culture (74.4%) were positive and 11 (25.6%) showed negative results, and were therefore excluded from the study. Using HSP70-specific primers, 1962 bp and 1152bp bands were observed for HSP70 of L. major in Chabahar and L. tropica in Mashhad, respectively. Based on the results, there were 18 nucleotide differences between HSP70 of L. major in Chabahar and L. tropica in Mashhad.
Conclusion: Due to the morphological similarities between Leishmania species and inability to differentiate species through parasitological methods, the HSP70 gene can be used for identification of the species, and prevention and treatment of the disease.
Davoodi Jabber , Reza Norian , Mohammad Jalilvand ,
Volume 11, Issue 2 (Mar-Apr 2017)
Abstract
ABSTRACT
Background and Objectives: Ivermectin is an anti-parasitic medication frequently used in many food-producing animals. This study aimed to investigate the level of ivermectin residue in liver samples collected from slaughterhouses in Qazvin Province, Iran.
Methods: Overall, 88 bovine liver samples were randomly collected and analyzed for detection of ivermectin residues. The samples were analyzed for ivermectin contamination by enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC). The samples were extracted using liquid-liquid extraction procedure for the ELISA. Solid phase extraction using a C18 column followed by fluorescence-derivatized with 1-methylimidazole and trifluoroacetic anhydride in acetonitrile were used for the HPLC assay. Recovery values obtained from the HPLC method ranged from 81.3 to 92.5%, with a relative standard deviation of 6.7-12.2%.
Results: First, all samples were screened by the ELISA method. Based on the results, 16 samples (18.2%) contained no detectable levels of Ivermectin residue, while Ivermectin was found in 72 samples (81.8%). In addition, 22 of the positive samples (30.55%) contained high Ivermectin level (>50 ppb). Analysis of the samples by the HPLC method showed that eight samples (9.09%) contained ivermectin levels above the maximum residue limit.
Conclusion: This study demonstrates the presence of different levels of Ivermectin residue in bovine liver samples collected from the Qazvin Province in Iran. Therefore, regulatory authorities should ensure proper withdrawal period before slaughter of the animals.
Keywords: Ivermectin, Cattle, Liver, ELISA, HPLC.
