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Showing 3 results for Faezi Ghasemi
Isolation and Characterization of Burkholderia Cepacia Strains from Hospitalized Patients in the Hospitals of West Guilan Province
M Elmi Merian, M Faezi Ghasemi,
Volume 8, Issue 3 (Autumn[PERSIAN] 2014)
Abstract
Abstract Background and Objective: Burkholderia cepacia complex (BCC) is a plant pathogen that is an important mortality factor in immune-compromised and hospitalized patients. We aimed to Isolate and Characterize the Burkholderia Cepacia Strains from Hospitalized Patients in the Hospitals of West Guilan Province. Material and Methods: This study was conducted on 90 saliva and blood samples obtained from patients with blood infection, pneumonia, asthma, patients connected to the monitoring and ventilator systems, and immune-compromised patients in different sections of hospitals such as the pediatrics, internal section, ICU and CCU. Primary screening was performed by cultivating the samples on Burkholderia cepacia selective agar (BCSA) Sensitivity to antibiotics was tested by Kirby-Bauer and Muller-Hinton Agar (MHA) and the separated isolations were recognized by strengthening the gene rec A. Results: Of 90 isolated samples, only one strain suspected B. cepacia was isolated from 24-year old women with asthma. This strain was resistant to bacitracin, pipracillin and ciprofloxacin antibiotics. Conclusion: The incidence of B. cepacia is rare in western part of Guilan province, which is congruent with the results of overall incidence in Iran.
Antimicrobial Effect of Lactic Acid Bacteria against Common Pathogenic Bacteria
Mohammad Mohammaddoost Chakoosari, Mohammad Faezi Ghasemi, Alireza Masiha, Reza Kazemi Darsanaki, Abolfazl Amini,
Volume 9, Issue 5 (Nov,Dec-2015 2015)
Abstract

Abstract

      Background and Objective: Probiotics are living microorganisms that have beneficial effects on the health of digestive system. The aim of this study was to evaluate the antimicrobial ability of acidic and neutral supernatants (culture supernatant) of lactic acid bacteria against common bacterial pathogens.

       Methods: Four species of lactic acid bacteria (Lactobacillus plantarum PTCC1745, Lactobacillus PTCC1608, Lactobacillus Saki PTCC1712 and Lactobacillus Lactis PTCC1336) were obtained from the microbial collection of Iranian Research Organization for Science and Technology in Lyophilized form. The antimicrobial activity of neutral and acidic supernatants against bacterial pathogens was investigated using the Disk and Well Diffusion Agar methods.

      Results: Lactic acid bacteria showed good antimicrobial ability against six pathogenic bacteria with the highest inhibitory effect observed in Lactococcus lactis against E. coli PTCC1399 through well method with an average diameter of 14 mm inhibition zone. In this study, the well diffusion method was far more sensitive compared to the disk method and acidic supernatants showed higher antimicrobial efficiency compared to neutral types.

      Conclusion:  the Metabolites produced by lactic acid bacteria are able to inhibit the growth of pathogenic bacteria that can be an important and practical solution for the prevention and treatment of infections and ultimately improve human health.


Prevalence of blaoxa-1 and blashv Genes in E. coli Isolates from Hospitalized Patients in Rasht
Mohammad Faezi Ghasemi , Seyede Negin Dibadji,
Volume 10, Issue 5 (Sep-Oct-2016 2016)
Abstract

ABSTRACT

      Background and Objective: Extended-spectrum beta-lactamases (ESBL) are widely produced by Escherichia coli strains. The aim of this study was to determine frequency of blaOXA-1 and blaSHV genes in E.coli strains isolated from patients hospitalized in city of Rasht, Iran.

       Methods: In this cross-sectional study, 200 samples were collected from patients. The E. coli strains were identified using morphological characteristics and biochemical tests. Antimicrobial susceptibility testing was performed. The prevalence of the blaOXA-1 and blaSHV genes in the E. coli isolated was assessed by PCR method.

       Results: Overall, 160 E. coli strains were isolated. Of these, 83 (51.9%) showed ESBL activity while 71 (48.1%) did not. All positive strains were resistant to cephalothin. Moreover, 98.8% of ESBL-producing strains were resistance to amoxicillin, cefotaxime and ceftriaxone. The prevalence of the blaOXA-1 and blaSHV genes in ESBL-producing strains were 45% and 17%, respectively. In addition, 28.9% of the strains had both genes while the genes were absent in 9.6% of the strains.

      Conclusion: Prevalence of the blaOXA-1gene is higher than that of the blaSHV gene. The absence of both genes in some isolates indicates the possible role of other genes in the ESBL activity.

      Keywords: Prevalence, ESBLs, Escherichia coli, blaOXA-1gene, blaSHV gene.


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