Showing 6 results for Ghorbani
H Davoodi, S R Hashemi, H F Seow, M Ghorbani,
Volume 3, Issue 2 (Autumn – Winter 2010[PERSIAN] 2009)
Abstract
Abstract Background and objectives: Paraffin-embedded tissues and clinical samples are a valuable resource for molecular genetic studies, but the extraction of high-quality genomic DNA from this tissues is still a problematic issue. In the Present study, the efficiency of two DNA extraction protocols, a commercial kit and a traditional method based on heating and K Proteinase was compared. Material and Methods: Fifty paraffin-embedded blocks of colon cancer tissues (more than 5 years old) were used to compare two methods of DNA extraction. DNA was extracted by traditional method using heat and commercial DNA extraction (Qiagen kit) method. Then the purity and concentration of extracted DNA were measured by Spectrophotometer. Two sequences of TLR4 “The most important receptors in innate immunity” were amplified by polymerase chain reaction. SH-1 ‘188bp’ and SH-2 ‘124bp’ were amplified and then the products were separated on a 2% agarose gel. Results: The results show that the yield of DNA by traditional method (297 mg/ml) is significantly (p<0.01) higher than Commercial kit (176mg/ ml). But traditional method has the lower OD ratio (1.2) Compared to Commercial method. The Amplification of the TLR4 gene sequences is more successful by the traditional method (p<0.01) compared with commercial method. The length of the sequence affects on the results of PCR in that short sequence is amplified more successful compared to the long sequence. Conclusion: The traditional method is more successful in PCR amplification and also simple and cheap. Therefore, we recommend using this method for DNA extraction taken from the paraffin-embedded blocks with more than 5 years old and selecting shorter sequence for better amplification in PCR. Key words: DNA Extraction, paraffin embedded tissue, PCR
B Rahimi-Esboei, Sh Gholami, A Ghorbani Pasha Kolaei, M Pour Haji Baqer, H Hasannia, R Shaban, As Paqeh,
Volume 7, Issue 2 (summer[PERSIAN] 2013)
Abstract
Abstract
Background and Objective: Nowadays, downsizing the government to have an effective and flexible organization is considered to be government’s top priority in the world and outsourcing is one of the ways to achieve this goal. Accordingly, Shahid Beheshti University of Medical Sciences has delegated some of its hospitals' duties to the private sectors. The present study has been carried out to assess the performance of outsourced clinical laboratories.
Material and Methods: This Descriptive Evaluation study was carried out during 6 month (May 2011 till February 2012). The data was collected by two questionnaires with close-ended questions and one with open-ended questions, and analyzed by Wilcoxon, using SPSS software.
Results: There is an improvement in outsourcing laboratory services. For example, increase in the number of tests (32%) and in the type of tests (37%). Consequently, increase of hospital income (51%). The number of personnel is decreased and their responsibility and behavior are improved.
Conclusion: Overall, it seems that outsourcing laboratory program could achieve its major goals such as: Absorbing non-governmental resources in both administrative and financial aspects, omitting extra expenditure, acquiring benefits, and upgrading productivity of laboratories.
Key words: Outsourcing Laboratory Peformance
B Rahimi-Esboei, M Fakhar, A Ghorbani, M Pour Haji Baqer, As Paqeh, P Shahnasi, M Rezaei,
Volume 7, Issue 2 (summer[PERSIAN] 2013)
Abstract
Abstract
Background and Objective: Cercarial dermatitis (CD) or swimmer’s itch is a severe inflammatory reaction caused by penetration of the skin by avian and herbivorous schistosomes, esp genus Trichobilharzia. Since both intermediate and final host of this parasite exist in North of Iran, we aimed at determining the prevalence of cercarial dermatitis among paddy-field workers in Central areas of Mazandaran Province.
Material and Methods: To perform this descriptive-cross sectional study, we refer to bird refuges of migrating water and Paddy-field around man-made ponds of Babol, Sari, Ghaemshahr and Savadkoh districts. The Hand and foot of the farmers were examined clinically for detection of infected people and their signs and symptoms were recorded on a sheet of paper.
Results: Based on the results, 77.5 % of the workers suffer from CD. All of them are males and the majority of them are indigenous.
Conclusion: High prevalence of Cercarial dermatitis among paddy-field workers led to a health dilemma. Thus, by increasing awareness of farmers, implementing suitable approaches, controlling the disease and sanitizing the contaminated areas can promote health situation.
Key Words: Trichobilharzia Avian Schistosomiasis Cercaria Dermatitis Paddy-Field Workers Itch
Mojtaba Raeisi , Fatemeh Ghorbani Bidkorpeh , Mohammad Hashemi , Bektas Tepe , Zahra Moghaddam , Masoud Aman Mohammadi, Seyyed Mohammad Ali Noori ,
Volume 13, Issue 2 (Mar-Apr 2019)
Abstract
ABSTRACT
Background and objectives: Utilization of essential oils instead of chemical preservatives has received significant attention in recent years. The present study aims to evaluate chemical composition and antibacterial and antioxidant properties of essential oils of Zataria multiflora, Artemisia deracunculus and Mentha piperita.
Methods: Chemical profile of the essential oils was analyzed by gas chromatography/mass spectrometry. The microwell dilution and agar disk diffusion methods were used to evaluate the antibacterial properties of the essential oils. Total phenolic content, β-carotene-linoleic acid bleaching test and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays were carried out to determine the antioxidant properties.
Results: Menthol (39.18%) and mentone (21.64%) were the main components of the essential oil of M. piperita, while estragol (34.75%) and limonene (15.72%) were the major components of the essential oil of A. dracunculus. The main components of the essential oil of Z. multiflora were carvacrol (36.81%) and thymol (33.04%). The essential oils of M. piperita and Z. multiflora showed greater antimicrobial effects. Moreover, Z. multiflora showed the greatest antioxidant activity among the essential oils. The total phenolic content of Z. multiflora was 228.14±0.45 mg gallic acid equivalent/g.
Conclusion: Given their favorable antioxidant and antimicrobial properties, the essential oils of Z. multiflora, A. deracunculus and M. piperita can be used as natural food preservatives.
Keywords: Zataria multiflora, Artemisia deracunculus, Mentha piperita, antibacterial effect, antioxidant effect.
Zahra Ahmadnia , Samane Rouhi, Hamed Mehdinezhad , Siamak Sabaghi , Alireza Firouzjahi , Mohammad Ranaei , Hossein Ghorbani , Maryam Pourtaghi, Mana Baziboron ,
Volume 18, Issue 4 (Jul-Aug 2024)
Abstract
Pasteurella species are one of the most common pathogenic bacteria in domestic animals, and they are seen more in people with weak immune systems. This research aims to investigate a case of a patient with multiple sclerosis from whose sputum Pasteurella multocida (P. multocida) was isolated. The patient was a 28-year-old man with multiple sclerosis who had persistent coughs due to food being stuck in his throat. The patient was a 28-year-old man with multiple sclerosis who had persistent coughs due to food being stuck in his throat. The primary diagnosis was pneumonia hydropneumothorax and complete collapse of the left lung. The patient's sputum culture after the first visit to the hospital was positive for P. multocida, which was not found in a second culture. In the subsequent cultures of the patient, Acinetobacter, Klebsiella, Pseudomonas aeruginosa (P. aeruginosa), and Citrobacter were found, which had extensive drug resistance to all antibiotics. In the secondary computerized tomography (CT) scan, mild pleural effusion on the left side, pneumothorax, and complete collapse with bronchiectasis was seen. Despite the treatments, the patient finally died of cardiac arrest and bradycardia. Infection with P. multocida was found in a patient with multiple sclerosis. Also, hospital-acquired infections with drug resistance caused by the weakness of the patient's system appeared in the patient who was hospitalized in the intensive care unit (ICU), and finally, the patient died. According to antibiotic patterns, the best antibiotic to which the bacteria is sensitive can be considered the primary treatment to avoid irrational antibiotic prescriptions.
Noori Noha Alsharifi , Mahin Gholipur , Somayeh Ghorbani , Fatemeh Mohammadzadeh , Safoura Khajeniazi ,
Volume 18, Issue 5 (Sep-Oct 2024)
Abstract
Background: Tumor necrosis factor alpha (TNFα) is a 17 kDa, an important soluble pro-inflammatory cytokine, which is involved in some tissue dysfunctions, including thyroid and liver tissue. In spite of its role in thyroid and tissue damage separately, the relationship between this factor and these two disorders has not been clarified. The aim of the present study was to evaluate liver biochemical parameters and TNFα in hypothyroid patients compared to euthyroid subjects.
Methods: To achieve this purpose, samples were transferred into tubes without anticoagulants and then centrifuged immediately to separate the serum. All markers in the serum were measured using commercial kits, including T3, T4, TSH, and TNFα, which were detected using the ELISA method. Liver function tests, including albumin, total bilirubin, and total protein were measured by spectroscopy and the colorimetric method, respectively. In addition, AST, ALT, ALP, and GGT were detected using enzymatic methods.
Results: Our results showed that the level of TNFα in hypothyroid patients was significantly higher than that in normal individuals (P = 0.009). TNFα had a significantly positive correlation with TSH and T3 but a negative correlation with T4. Furthermore, AST, ALT, and GGT had a positive correlation with TSH and a negative correlation with albumin, total protein, and total bilirubin. These correlations were insignificant (P < 0.05).
Conclusion: According to our data, the positive correlation of TSH with both TNFα and liver function tests may indicate a relationship between thyroid and liver function with each other.
