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Showing 2 results for Karbasizade, V
Antimicrobial Resistance to Ceftazidime and Ceftriaxone, and Detection of TEM Gene in Esherchia Coli
Jahani, S, Shahreki Zahedani, Sh, Karbasizade, V,
Volume 8, Issue 4 (supplement Issue[PERSIAN] 2015)
Abstract

Abstract Background and Objective: In the past, most strains of E. coli were susceptible to a wide range of antimicrobial agents, but this situation is now changed by indiscriminate use of antibiotics. Ceftriaxone and Ceftazidime are the most current antibiotics used for Enterobacteriaceae infections in hospitals. The aim of this study was to determine antimicrobial resistance of Escherichia coli strains isolated from patients. Material and Methods: During a 12-month period, 200 clinical samples taken from patients referred to Zahedan hospitals were assessed to isolate Escherichia coli. Antibiotic susceptibility was determined by disk diffusion method and micro-broth dilution and Bla TEM resistance genes were detected by PCR. Results: Following phenotype verification testing, 112 isolates (56%) were produced Extended Spectrum Beta Lactamase (ESBLs) and 130 isolates were potential producers of beta-lactamase (ESBL). Using PCR, 72 isolates (38.55%) have TEM gene. Conclusion: The rate of antibiotic resistance of Escherichia coli isolates to ceftriaxone and ceftazidime is high therefore, it seems reasonable to do antibiogram before treatment. Keywords: Extended-Spectrum-Beta-Lactamase, Esherchia coli, Disc Diffusin, Micro-Broth Dilution


A Survey of Frequency and pattern of Methicillin-Resistant Staphylococcu s aureus Isolated from nosocomial infections
Behshood, P, Karbasizade, V, Naghavi, Ns,
Volume 9, Issue 2 (may,jun 2015[PERSIAN] 2015)
Abstract

Abstract

Background and Objective: Methicillin-resistant Staphylococcus aureus (MRSA) is a major pathogen involved in nosocomial infections. Because of increasing antibiotic resistance of these strains, treatment of these infections has become very difficult. This study aimed to determine the frequency and drug resistance pattern of MRSA isolates from nosocomial infections in hospitals.

Material and Methods: the isolates of S.aureus (n= 100) isolated from clinical samples such as: urine, blood, wound, throat, sputum, cerebrospinal fluid, catheter and other purulent discharge from in patients were identified using biochemical tests. MRSA strains were isolated by using agar screening method and then drug resistance pattern of them was determined by disk diffusion method.

Results: Out of 100 S.aureus strains, 65 (65%) were MRSA. Drug resistance of MRSA isolates to most antibiotics were high: penicillin100%, oxacillin 100%, nitrofurantoin 80%, tetracycline 63%, erythromycin 58.4%, gentamicin 46.1%, clindamycin 33.8%, cotrimoxazole 35.3% and ciprofloxacin 26.1%. Also 35 of MRSA isolates were multiple drug resistance (MDR).

Conclusion: The prevalence of MRSA isolates and also their resistance to other antibiotics were high.

Keywords: Drug Resistance, Methicillin-Resistant Staphylococcus Aureus, Multi-Drug Resistant


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