Background: Type 2 diabetes mellitus (T2DM) is a non-communicable disease, manifesting hyperinsulinemia, insulin resistance, hyperglycemia, and low-grade chronic inflammation associated with various micro and macrovascular complications. The present study aimed to estimate vitamin D (Vit D) levels, total antioxidant capacity, and malondialdehyde (MDA) levels in T2DM patients compared with healthy individuals. In addition, we assessed Vit D, total antioxidant capacity, and MDA levels in patients with T2DM and their association with HbA1c, insulin resistance and lipid profile parameters.
Methods: Seventy patients with T2DM aged 35 to 50 years were selected and 70 healthy age-matched subjects were selected as controls. Serum Vit D and insulin were estimated by the enzyme-linked immunosorbent assay (ELISA). Glycosylated hemoglobin (HbA1C) was assessed by high-performance liquid chromatography (HPLC) method and other routine lipid profile investigations were carried out using a Beckman Coulter fully automated analyzer.
Results: Vitamin D levels significantly decreased in T2DM patients. HbA1C and insulin resistance values are significantly increased in type 2 diabetic patients. Vitamin D levels negatively correlated with MDA, insulin resistance, and HbA1c, while positively correlated with total antioxidant capacity. Nevertheless, there is no significant correlation between lipid profile parameters.
Conclusion: Vitamin D deficiency may be one of the vital risk factors responsible for increased oxidative stress in patients with T2DM. Regular monitoring and supplementation of Vit D are beneficial for the reduction of oxidative stress and vascular complications in these patients.
