Kargar, M, Ebrahimi, E, Amini, J, Najafi, A, Kheirkhah, B,
Volume 8, Issue 4 (supplement Issue[PERSIAN] 2015)
Abstract
Abstract
Background and Objective: Listeria monocytogenes is a bacterium transferred by foods and is the agent of many sporadic and epidemic diseases in humans. This study aimed to investigate the prevalence of L. monocytogenes and to determinine their antibiotic resistance profile in red meats.
Material and Methods: this cross-sectional study was performed on 400 red meat samples obtained from industrial slaughterhouses placed in Kerman, Iran. First, the samples were enriched with Simultaneous Enrichment Broth (SEB), and then plated onto Palcam agar and Tryptic Soy Broth Yeast Extract Broth (TSAYE). After identification of the isolates based on biochemical tests and PCR, the isolates were checked for their antibiotic resistance profile using disk Diffusion
Results: of 400 samples, 12 samples (3%) were contaminated with different species of Listeria. Using PCR, hly gene was recognized in eight samples (2%) of L. monocytogenes. Furthermore, there was a significant difference in isolation rate of lamb samples compared to cow ones. While all of the isolates were resistant to clindamycin, amikacin and chloramphenicol, they were sensitive to penicillin.
Conclusion: in spite of low rate of infection in red meat samples in Kerman city, due to high risk of Listeria contamination in red meats, we recommend applying a routine screening to identify this bacterium in our county.
Keywords: Listeria Monocytogenes, Hly Gene, Red Meat, Antibiotic, Kerman
Shakerian, A, Sharafati-Chaleshtori, R, Karshenas, Aa, Rahimi, E,
Volume 9, Issue 3 (Jul,Aug2015[PERSIAN] 2015)
Abstract
Background and Objective: Cryptosporidium parvum is a zoonotic protozoan parasite causing diarrheal cryptosporidiosis. Numerous outbreaks of cryptosporidiosis have been reported worldwide. The transmission via milk, water and raw animal products is one of the important ways. The aim of this study was the identification of hsp70 gene in Cryptosporidium parvum in raw cow’s milk samples.
Material and Methods: In this cross sectional study, 38 raw cow’s milk samples of bulk tank were randomly collected from traditional and semi industrial cattle farms in Isfahan. To identify the protozoa in milk samples, the extracted DNA was evaluated by Nested polymerase chain reaction (PCR).
Results: Based on Nested polymerase chain reaction, 2 samples (5.26%) were infected to Cryptosporidium parvum.
Conclusions: The contamination of milk with Cryptosporidium Parvum is less than that of the other foodstuffs. Thus, it is necessary to reduce food contamination and to have appropriate health education programs.
Keywords: Cryptosporidium Parvum, Milk; Polymerase Chain Reaction.
