Showing 2 results for siddiqui
Imran Ahmed Siddiqui, Sowmya Gayatri C, Swati Suravaram, Bharat Kumar Reddy, Dhanalakshmi A,
Volume 18, Issue 2 (Mar-Apr 2024)
Abstract
Background: ‘M’ proteins or paraproteins refer to immunoglobulins that are produced by clonal plasma cells and are a characteristic feature of monoclonal gammopathies. Routine electrophoresis on agarose gel and immunofixation can be used to detect immunoglobulin paraprotein (M-protein). We aimed to evaluate the performance of agarose gel electrophoresis alone and in combination with immunofixation for detecting serum M-proteins.
Methods: One hundred and twenty-three patients suspected of paraproteinemia were evaluated. Routine serum protein electrophoresis (SPE) and immunofixation electrophoresis (IFE) protocols were performed. Data from SPE, and SPE-IFE (gel images and electrophoretograms) were collected and reviewed.
Results: 21% cases were confirmed using the SPE-IFE combination, and among them, 33% had positive light chain (λ) only on IFE. Similarly, nine cases with biclonal gammopathy on SPE were characterized by IFE.
Conclusion: IFE can be a confirmatory test in cases where SPE results are not reliable and it can be a complementary test when characterization of the M protein detected on SPE is required.
Sahar Siddiqui, Mohd Suhail Lone, Umer Qureshi, Rayees Khanday,
Volume 19, Issue 2 (3-2025)
Abstract
Background: Staphylococcus aureus (S. aureus) is a common pathogen that causes both community and hospital-acquired infections Objectives: To identify clinico-microbial profiles of S aureus acquired in the community and in the hospital. Methods This study was conducted from January 2021 to December 2022 in Postgraduate Department of Pediatrics Children Hospital Srinagar J&K on patients aged one month to 18 years suspected of having S. aureus sepsis or disseminated disease. Results: There were 56 patients identified with S aureus septicemia (SAS) over the study period; 37(66.10%) had community acquired S aureus sepsis (CASS) and 19(33.90%) hospital acquired S aureus sepsis (HASS). 35(62.50%) affected belonged to male gender; 40(71.43%) patients belonged to rural area. Localized musculoskeletal symptoms (91.90%) were the most commonpresentation P value < 0.05. Pleuropulmonary disease and necrotizing soft tissue disease were significantly associated with patients of HASS and CASS respectively. The common disease presentation was pneumonia followed by abscess. Septic shock, respiratory failure, multi organ dysfunction, etc were the common complications seen more often in HASS. Out of 50 patients who had SAS in cultures, 48 were Methicillin resistant S aureus (MRSA) and 2 were Methicillin sensitive S aureus (MSSA). The survival rate from CASS was 94.60% and from HASS was 89.50% Conclusions: CASS and HASS affects children, is multifocal, and has high morbidity associated. The sensitivity pattern of Staphylococcus aureus in our settings as well as globally has undergone a dramatic change with rise in proportion of Methicillin resistance and needs to been addressed urgently.
