Vahid Zolghadri, Alireza Barari, Asieh Abbasi Daloii, Hosein Abed Natanzi,
Volume 19, Issue 5 (9-2025)
Abstract
Background and objectives: Melanoma is an aggressive and malignant cancer that results from the transformation of pigment-producing melanocytes. The aim of this study was to investigate the effect of nettle extract consumption and aerobic exercise on gene expression IFN-γ and Endostatin in melanoma cancer in liver tissue of mices .
Methods: 20 male BALB/c mices were randomly divided into 4 groups including: control, endurance, nettle, endurance+nettle. The training program included running on a treadmill for 30 minutes at a speed of 16 meters per minute. One meter per minute was added every week until it reached 22 meters per minute in the eighth week. Melanoma cells were induced subcutaneously in the left side of the mouse. The experimental group consumed 30 mg/kg /day of nettle ethanol extract orally for 8 weeks. RT PCR was used to measure the expression of IFN-γ and Endostatin genes.
Results: IFN-γ gene expression in experimental groups was not different from control group; While the level of Endostatin was significantly reduced (P = 0.142, P <0.001, respectively). IFN-γ gene expression levels in the experimental groups increased compared to the control group, but did not reach a significant level. Also, Endostatin gene expression levels in training and combination groups were significantly reduced compared to the control group (P = 0.022, P <0.001, respectively).
Conclusion: The results showed that endurance training with nettle extract may inhibit angiogenesis and capillary tissue formation in tumor tissue of mices with melanoma cancer by increasing IFN-γ and decreasing Endostatin.
