Parisa Hasanein , Mehrnush Sotudeh , Mousa Bohlooli , Mohamad Hadadi ,
Volume 19, Issue 6 (11-2025)
Background: DNA glycation damages DNA by inducing strand breaks, mutations, and ultimately changes in gene expression, which is considered a main factor in the pathogenesis of diabetes and its complications. Therefore, antiglycation agents have become the focus of recent research for preventing and alleviating diabetes complications. According to the reported antidiabetic effects of Artemisia sieberi (A. sieberi) leaf extract, this study aimed to determine the effect of the ethanolic extract of A. sieberi on glucose-mediated DNA glycation for the first time.
Methods: DNA was incubated with glucose in the presence or absence of A. sieberi for 4 weeks. The inhibitory or facilitatory effects of A. sieberi on DNA structural changes were studied by various techniques. These techniques included UV–Vis, fluorescence spectroscopy, circular dichroism (CD), and agarose gel electrophoresis.
Results: The findings of UV–Vis and fluorescence spectroscopy showed that A. sieberi decreased DNA-AGE (Advanced glycation end products) formation. Based on the CD and agarose gel electrophoresis results, the structural changes of glycated DNA were decreased in the presence of A. sieberi.
Conclusion: Thus, A. sieberi has beneficial effects against DNA glycation and could be a promising agent for ameliorating the adverse effects of glycation in the presence of glucose and in conditions of raised blood glucose, such as diabetes, after confirmation in further studies.
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