Showing 4 results for Nitric Oxide
M Kadkhodaei Elyaderani, M Rostami, B Keikhaie, M Pedram,
Volume 4, Issue 1 (4-2010)
Abstract
73/ Spring summer 2010, Vol.4, No. 1 Medical Laboratory Journal Correlation between Nitric oxide (NO) & Asymmetric dimethylargininie (ADMA) Hemoglobin Concentration in sickle cell patients Abstract Background and objectives: The importance of Nitric oxide (NO) and Asymmetric dimethylargininie (ADMA) in pathophysiology of Sickle cell disease (SCD) is being increasingly clarified. Since very few of the studies have been conducted in the word and no study has been carried out in Iran, especially in Khuzestan province where is the main center of Sickle Cell disorder (SCD) in Iran, We decided to conduct the present study. Material and Methods: EDTA anticoagulated plasma samples were obtained from 35 healthy controls (Hb AA), 35 heterozygous (HB AS) and 35 homozygous (HB SS) sickle cell anemia patients. Plasma concentration of NO was measured by Colorimetric and Griess reaction and the concentration of ADMA by employing ELISA method. Then the results were analyzed by tstudent test and OneWay ANOVA. Results: There is a positive significance correlation between Hemoglobin (Hb) and NO in SS (r=0.703) and AS (r=0.366) groups. Also, a negative correlation between Hb and ADMA in SS (r=-0.786) and AS (r=-0.478) groups is seen. No correlation is found between these parameters in AA group. Conclusion: The prevention of Hb concentration decrease and prescription of NO donors and (or) ADMA disintegrators can be helpful for improving clinical signs of sickle cell patients. Key words: Nitric oxide (NO), Asymmetric dimethylargininie (ADMA), Sickle cell disease (SCD). Kadkhodaei ElyaderaniM (PhD) Associateprof. of Biochemistry, Dept of Clinical Biochemistry and Research Center of Thalassemia & Hemoglobinopathy, School of Medicine , Jundishapur University of Medical Sciences, Ahwaz , Iran Rostami M (MSc) MSc of Biochemistry, Dept of Clinical Biochemistry, School of Medicine , Jundishapur University of Medical Sciences, Ahwaz , Iran . Keikhaie B (PhD) Assistant Professor of Pediatric Hematology and Oncology, Shafa Hospital and Research Center of Thalassemia& Hemoglobinopathy, Jundishapur University of Medical Sciences, Ahwaz, Iran. PedramM (PhD) Professor of Pediatric Hematology and Oncology, Shafa Hospital and Research Center of Thalassemia & emoglobinopathy, Jundishapur University of Medical Sciences, Ahwaz, Iran. Corresponding: Kadkhodaei ElyaderaniM Email: Kadkhodaeim@yahoo.com
M Rahbani-Nobar, Mh Somi, A Fattahi, N Dolatkhah, M Nourazarian, S J Seyedi-Khoshknab, B Pourasghari, H Dolatkhah,
Volume 4, Issue 2 (10-2010)
Abstract
Abstract Bachground and objectives: Epidemiological studies have shown that using tobacco products is one of the main factors in forming malignancies in various tissues of the body. There is more than 600 μgr nitric oxide radical (NO°) in gas phase in each cigarette with fresh smoke. Hence, oxidation of nitrogen components in tobacco, more than 100 μgr of atmospheric NO°is produced by smoking, would be transferred to the body without any filtration. We studied nitric oxide levels in the gastric juice of smokers and non-smokers patients with active peptic ulcer. Material and Methods: In this study, 43 smoker patients with active peptic ulcer (14 female & 29 male) referred to gastroenterology clinic with mean age of 45.30±13.16 as case group.Forthy-three non-smokers without peptic ulcer (13 female & 30 male) aged 42.67±16.04, 43 smoker without peptic ulcer (16 female & 27 male) with mean age of 44.58±12.07 and 43 non-smoker with active peptic ulcer (20 female & 23 male) with mean age of 45.37±13.39 were selected as control groups of 1, 2 and 3 ,respectively. The level of Nitric oxide in gastric juice was measured by using Greiss colorimetric method. Results: Comparing with control group one and two, meaningful rise is noticed in mean level of nitric oxide case group (p<0.0001). Mean levels of NO in control group 1, 3 and case group are 4.21±1.13, 5.37±2.26, 7.90±2.12 μmol/L, respectively. Nitric oxide level in case group in comparison with control group 2 dose not show Significant difference (p=0.656). Mean levels of NO in control 2 and case groups are 7.45±1.54 and 7.90±2.12 μmol/L, respectively. Conclusion: It can be concluded that cigarette smoking may be one of the cause of increased level of gastric juice nitric oxide. This increase may be due to component in cigarette smoke and tar. These components can cause DNA damage through oxidation-reduction cycle and consequently increase the risk of malignancies in gastric tissues. Key words: Cigarette Smoking, Nitric Oxide, Nitrosative Stress, Active Peptic Ulcer
N Puramini, A Movahedian, S Bordbar-Bonab, E Fattahi, A Mirza-Aghazadeh, A Bahrami, Sh Fattahi, H Dolatkhah,
Volume 8, Issue 5 (1-2015)
Abstract
Abstract Background and Objective: Recently, diabetes mellitus has been known as one of the main cause of upper gastrointestinal symptoms. Since a high prevalence of H. Pylori in diabetic patients has been reported, we aimed to evaluate the level of gastric juice Nitric Oxide (NO°), Oxidative Stress and Glycated Hemoglobin. Material and Methods: In case group, the participants were 60 diabetic patients infected with H. Pylori, and in control groups 60 diabetic patients without H. Pylori and 60 healthy individuals. The level of NO° in gastric juice was measured calorimetrically and the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX) in gastric biopsy was determined using standard methods. The percentage of Glycated Hemoglobin (HbA1C) was measured by ion exchange chromatography. Results: In case group compared to controls, significantly increased level of blood HbA1C, nitric oxide in gastric juice, activity of SOD and GPX in the gastric mucosa were observed (p<0.0001). Conclusion: A significant increase of glycated hemoglobin in diabetic patients with H. Pylori and high activity of antioxidant enzymes in the case group may indicate a high production of reactive oxygen species and the presence of oxidative stress in these patients. Key Words: Diabetes Mellitus, H. Pylori Infection, Glycated Hb, Nitric Oxide, Oxidative Stress
Abdolhamid Zokaei, Mehran Ghahramani, Golbano Bolouri, Mahdieh Nassiri Avanaki,
Volume 17, Issue 2 (3-2023)
Abstract
Background and objectives: Considering the importance of aging and the associated physiological changes, as well as the effects of exercise on angiogenesis and cardiac index, this study aimed to compare continuous and periodic exercise in form of high-intensity interval training (HIIT) on serum levels of nitric oxide (NO) and vascular endothelial growth factor (VEGF) in old rats.
Methods: In this study, 30 old male rats were randomly divided into three groups: continuous training (n=10), HIIT (n=10), and control group (n=10). Interventions were performed for 8 weeks. To evaluate the research variables, 72 hours before the first training session and after the last training session, 3 ml of blood were taken from the tails of the rats. One-way analysis of variance was used to analyze the findings and Levene's test was used for assessing the homogeneity of variance. All statistical tests were performed using SPSS 17 software at a significance level of 0.05.
Results: Both training exercises significantly increased NO and VEGF levels compared to the control group (p<0.05).
Conclusion: The results of this study showed that 8 weeks of continuous and interval training cause a significant increase in the level of angiogenic factors in old rats. Therefore, these exercises and especially alternative exercises can be used as a suitable way to increase angiogenesis in the elderly.
