Mohaddeseh Abouhosseini Tabari , Mohammad Reza Youssefi, Bibi Razieh Hosseini Farash , Mohammad Amin Ebrahimi, Niki Nabavi Mousavi , Elham Moghaddas,
Volume 11, Issue 5 (9-2017)
Abstract
ABSTRACT
Background and Objectives: Due to adverse effects of pentavalent antimonial treatment of visceral leishmaniasis using herbal medicines has received a lot of attention. In this study, we evaluated inhibitory activity of Pelargonium roseum essential oil on growth of Leishmania infantum promastigotes, and compared it with that of Glucantime.
Methods: Inhibitory effects of different concentrations (1, 2.5, 5, 10, 25, 50 and 100 µL/ml) of P. roseum essential oil on the growth of L. infantum promastigotes were studied by MTT assay after 24 and 48 hours. All the data were analyzed by one-way analysis of variance. The effect of the essential oil was later compared with that of standard dose of Glucantime.
Results: After 24 hours, different concentrations of the essential oil had no significant inhibitory effect on promastigotes of L. infantum. After 48 hours, all concentrations except for 1µL/ml showed significant anti-leishmanial activity. In addition, the lowest concentration that had significant inhibitory effect on L. infantum promastigote was 5 µL/ml.
Conclusion: The essential oil of P. roseum has favorable inhibitory activity on the growth of L. infantum promastigotes after 48 hours. This indicates the therapeutic potential of this plant for treatment of leishmaniasis.
Keywords: Leishmania infantum, Pelargonium, Promastigote, Meglumine antimoniate.
Mojtaba Raeisi, Kamal Mirkarimi, Behrooz Jannat, Bahman Rahimi Esboei, Abdol Sattar Pagheh, Zahra Mehrbakhsh, Fatemeh Ghaffarifar, Oghlniaz Jorjani, Masoud Foroutan,
Volume 14, Issue 4 (7-2020)
Abstract
Background and objectives: Leishmaniasis is a tropical disease caused by protozoan parasites from the genus Leishmania. In this study, we aimed at investigating the in vitro anti-leishmanial effect of essential oils of Rosmarinus officinalis, Mentha pulegium, Foeniculum vulgare, Lippia citriodora and Pelargonium graveolens.
Methods: The essential oils were prepared from freshly dried and powdered plants with steam-distilled water. Iranian strain of Leishmania promastigotes was cultured in RPMI medium and the inhibitory effects of different concentrations (25, 32, 62.5, 125, 250, 500 and 1000 μg/ml) of the essential oils were investigated at 24, 48 and 72 hours. The number of live parasites before and after treatment with the essential oils was counted by trypan blue 10% staining and using neobar lam.
Results: The essential oils significantly decreased the number of promastigotes in a dose-dependent manner (P<0.05). However, the inhibitory effects of F. vulgare and R. officinalis essential oils were more profound compared to other essential oils. Moreover, concentrations of 500 and 1000 μg/ml of these two essential oils exerted equal and more anti-leishmanial potency compared to glucantime, the first-line drug used for treatment of leishmaniasis.
Conclusion: Based on the results, it is recommended to evaluate the in vivo anti-leishmanial effects of the tested essential oils, particularly F. vulgare and R. officinalis.
