Seyedeyn Khorasani, M, Yousefi Mashouf, R, Majlesi, A, Jaefari, M, Alikhani, My,
Volume 9, Issue 1 (4-2015)
Abstract
Abstract
Background and Objective: Helicobacter pylori (H. Pylori) infection is related to chronic gastritis, peptic ulcer, duodenal ulcer and gastric cancer. Thus, identification and treatment of the infection have a considerable importance. The aim of this study was to compare three methods of Histopathology, Culture and Rapid Urease test (RUT) in identification of H. Pylori in gastric biopsy specimens.
Material and Methods: The participants were 153 patients (64 women and 89 men) suffering from digestive complaints, who referred to the endoscopy department of Shahid Beheshi Hospital in Hamadan, Iran. Three gastric biopsy samples were collected from each patient and examined by standard RUT, Histopathology and culture methods for diagnosis of H. Pylori.
Results: Out of 153 patients, 69.9%, 27.4% and 2.6 % had gastritis, gastric ulcer and gastric cancer, respectively. The rate of infection with Urease test, culture and histology were identified 49.7%, 54.2%, and 89.5%, respectively. The sensitivity and specificity of the RUT result at first hour and after the first up to 24 hours were 55.4% and 80%, and 55.4% and 66/7%, respectively. The sensitivity and specificity of culture method were 60.6% and 100%, respectively.
Conclusion: Based on the results, Histopathology method has a more sensitivity than both Culture method and rapid urease test for diagnosis of H. Pylori, and RUT is more specific when done in the first hour rather than after the first hour.
Key words: Helicobacter pylori, rapid urease test methods, RUT, Histopathology
Mojtaba Chizari, Seyed Masoud Hosseini, Ali Mohammadi, Javad Fakhari, Mojtaba Mashhadi Mohammadzadeh-Vazifeh,
Volume 14, Issue 6 (11-2020)
Abstract
Background and objectives: Dried Black Curd (DBC), also known as Gharahghorut (Persian), is a dairy product produced from curd of yogurt or doogh. The aim of this study was to evaluate microbial contamination of industrially and traditionally produced DBC in Iran.
Methods: Four DBC brands holding certification of the Institute of Standards and Industrial Research of Iran (ISIRI), Iran Ministry of Health and Medical Education (IMHME) and ISO were purchased from a market in Tehran, Iran. Microbial monitoring for presence of coliform, Escherichia coli, coagulase-positive Staphylococcus aureus, mold and yeast was performed using different basic, enriched, selective and differential media including peptone water agar, MacCankey agar, blood agar and brain heart infusion agar, etc. according to the ISIRI standards (No.13299).
Results: None of the tested samples had microbial contamination. However, in one traditional DBC sample, the population of mold and yeast was higher than the acceptable level defined by the ISIRI standard.
Conclusion: We found no contamination with coagulase-positive S. aureus, E. coli and coliforms in four brands of traditionally and industrially produced DBC in Iran. This indicates that the hygienic practices designated by the IMHME are well-implemented in the industrial sector. However, the mold and yeast contamination in traditionally produced DBC should be prevented by applying hygienic practices during the process of manufacturing and distribution.
