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Comparison of Three Methods of Pour Plate (PP) ، Most Probable Number (MPN)and Membrane Filter (MF) for Detection of Escherichia coliin Well Water Samples in Tehran's Parks in 2010
Mm Soltan Dallal, M Hosseini, Tp Abedi Mohtaseb, A Tabatabaei Bafroei,
Volume 3, Issue 2 (10-2009)
Abstract
Abstract Background and objectives: Water-born diseases are typically caused by pathogens transmitted by orofecal way. Because it is no practical and no economical and also it is time-consuming to find water-born pathogens in water reservoirs, the laboratory studies are performed on the basis of indicator microorganism. Escherichia coli is considered as the most important indicator bacterium for water monitoring. The aim of this study was to evaluate the three methods of Pour Plate (PP), Most Probable Number (MPN) and Membrane Filter (MF) in isolation of Escherichia coli in well water of Parks. Material and Methods: One hundred and sixty five samples of well water, from five geographical zones of north, south, east, west and center of Tehran, were taken in a sterile condition and sent to microbiology department of health faculty to assess with three methods of PP, MPN and MF. The results were analyzed by chi-square. Results: The results indicate that 90 water samples (54.5%) aren’t health. The samples taken from south of Tehran are most contaminant than other zones. The highest contaminated Samples (54.5%) are related to membrane filtration method in comparison with MPN (34.5%) and PP (27.3%). Conclusion: Since the MF method can recognize the contaminants quickly and effectively, we recommend it more. Based on these results, it is essential to educate children not to drink well water in parks. Keywords: well water contamination, Escherichia coli, Tehran's parks
SeroepidemiologicalPrevalence of Helicobacter Pylori of the Patients Referred to the Central Laboratory in South of Tehran, 2010
A Choupani, Z Rostami, Aa, A Abdullahi,
Volume 7, Issue 1 (4-2013)
Abstract
Abstract Background and Objective: Helicobacterpylorus is the major cause ofinflammation andulcer instomach, and immunoglobulin IgG is one of the antibodies produced against it, which is important in the course and diagnosis of the previoussufferers. The awareness of the prevalence of this disease can be helpful for the physicians to choose the way of treatment. Material and Methods: In these cross-sectional study, 516patients referred to laboratory was studied. After separating the serum, Antibody Helicobacter pylori IgGtest wasdone by ELISA method. Results: of 516, 156 (30.2 %) of the patients have a positive result, 51(32.7%) are males and 105 (67.3 %) are females. Positive percent of males (43.5%) is greater than females (26.5%).Over-45-year-old women (9.8 %) have the highest percentage of disease titers. Conclusion: The percent of positive cases in men is more than that the women. Over-45-year-old women (9.8 %) have the highest percentage of positive case. Keywords: Helicobacter pylori IgG, Tehran, ELISA
Antibiotic Susceptibility of Staphylococcus aureus and Enterococcus Spp. Isolated from some Hospitals in Tehran
Razaz Rahmati, N, Mohabati Mobarez, A, Khoram Abadi, N, Sharifzade Peyvasti, V, Shokoohizade, L,
Volume 9, Issue 2 (7-2015)
Abstract

Abstract

Background and Objective: Vancomycin-resistant enterococci (VRE) and methicillin-resistant Staphylococcus aureus (MRSA) are the leading nosocomial pathogens in Iran. We aimed at evaluating the resistance patterns of Staphylococcus aureus and enterococci to the antibiotics recommended by Clinical and Laboratory Standards Institute (CLSI).

Material and Methods: Two-hundred forty Staphylococcus aureus and 203 Enterococcus isolated from urine, wound, blood, trachea, stool and other clinical specimens of inpatients and outpatients were obtain from some hospitals in Tehran. Sensitivity of S. aureus to oxacillin, vancomycin, chloramphenicol, nitrofurantoin, erythromycin, clindamycin and linezolid were determined by disk diffusion according to CLSI (M100-S22) guidelines. Likewise, sensitivity of enterococci to ampicillin, vancomycin, teicoplanin, tigecycline, linezolid, tetracycline, chloramphenicol and nitrofurantoin was invesditated by the same method.

Results: Of 240 S. aureus isolates, 56%, 46.6% and 40.4%were resistant to oxacillin, erythromycin and clindamycin, respectively. We found one S. aureus isolate with low   susceptibility to vancomycin (VISA; 0.5%). All S. aureus isolates were sensitive to chloramphenicol, nitrofurantoin and linezolid. Of 203 Enterococcus spp., 47.3%, 24.6%, 9.4%, 85.2%, 11.3% and 4.4% were resistant to ampicillin, vancomycin, teicoplanin, tetracycline, chloramphenicol and nitrofurantoin, respectively. All these isolates were sensitivity  to tigecycline and linezolid.

Conclusion: Resistance of S. aureus isolates to oxacillin, erythromycin and clindamycin was significant. Enterococci isolates were remarkably resistant to ampicillin, vancomycin, teicoplanin, tetracycline, chloramphenicol and nitrofurantoin. Given our results, we suggest vancomycin, chloramphenicol, linezolid and nitrofurantoin for S. aureus infections and tigecycline and linezolid as drugs of choice for enterocoocal infections treatment.

Keywords: Staphylococcus aureus, Enterococcus, Tehran, Hospitals


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