Showing 5 results for Urine
K Ghazisaidi, F Fateminasab, Sh Vatani, Y Azimi, S Bakhshandeh Nosrat, M Mohamadi,
Volume 2, Issue 1 (4-2008)
Abstract
Abstract Background and objectives: Ureaplasma urealyticum is one of the most important causes of Nongonococcal urethritis (NGU) and non-specific urethritis (NSU) in men. Also Mycoplasma hominis has a role in causing NGU and NSU. The aim of this study was the detection of Mycoplasma hominis & Ureaplasma urealyticum in men suffering from NGU and NSU by prostatic massage method and first- void Urine samples. Material and Methods: Urethral secretion samples, taken after prostatic massage and first-void urine, of 75 patients referred to department of bacteriology of Tehran University of Medical Sciences were examined for ureaplasma and mycoplasma by culture method in 2004-2005 Results: In prostatic samples, 19 and 11 samples are positive for ureaplasma and Mycoplasma, retrospectively. In first-void urine samples, 17 and 9 samples are positive for ureaplasma and Mycoplasma, retrospectively. Conclusion: The results show that if it is impossible to obtain urethral sample via prostatic massage , we can use first- void urine instead. Key words:Mycoplasma hominis, Ureaplasma urealyticum,Nongonococcal urethritis, Nonspecific urethritis, prostatic massage, first voided Urine
Hosseini, Sm, Farhang Ara, E, Yousefi Mashouf, R, Parsavash, S,
Volume 8, Issue 4 (1-2015)
Abstract
Abstract Background and Objective: The prevalence of different bacterial species and antibiotic resistance varies according to geographical conditions. Hence, we aimed to identify the prevalence of bacterial agents isolated from the urine culture and also investigate the antibiotic susceptibility of bacteria. Material and Methods: This cross-sectional study conducted on 7200 urine samples to identify the bacteria causing infections, using differential tests. The antimicrobial susceptibility was performed via disk diffusion method according to the Clinical and Laboratory Standards Institute (CLSI). Results: The most common bacteria were Escherichia coli (736 61.1%) and Klebsiella pneumonia (128 10.6%) and Enterococcus faecalis, (88 7.3%). The highest antibiotic susceptibility was related to Ciprofloxacin (174 14.45%) and the lowest to Amoxicillin (795 62.02%). Conclusion: Owing to the lowest resistance, it is recommended that Ciprofloxacin be used as the first line of treatment. In addition, Amoxicillin and Ampicillin should be used the least during primary treatment of urinary tract infections. Keywords: Antibiogram, Bacterial Resistance, Urine Culture
Mahsa Taherizadeh , Masoud Khoshnia , Sedighe Shams , Hamidreza Joshaghani ,
Volume 11, Issue 2 (3-2017)
Abstract
ABSTRACT
Background and Objectives: The incidence rate of esophageal cancer (EC) is high in north and northeast of Iran. Taurine is a sulfur-containing amino acid with a wide range of vital biological functions. The aim of this study was to compare plasma levels of taurine between patients with EC and healthy controls.
Methods: Plasma samples from 36 recently diagnosed cases of EC and 36 healthy adults were analyzed by high-pressure liquid chromatography. Data analysis was done using SPSS (version 16). The t-test was used to evaluate significant differences in the level of taurine between the two groups. P-values less than 0.05 were considered statistically significant.
Results: Patients with EC had significantly higher levels of plasma taurine compared with the controls (P<0.05).
Conclusion: Patients with EC have significantly higher levels of plasma taurine compared with healthy individuals. This finding suggests that the measurement of plasma taurine in patients with EC could be useful for the early diagnosis of the disease.
Keywords: Esophageal Cancer, Taurine, HPLC.
Maryam Moazeni, Mojtaba Nabili,
Volume 16, Issue 2 (3-2022)
Abstract
Background and objectives: The incidence of candiduria caused by Candida spp. has increased in recent years, particularly in hospitalized patients. Candiduria is most commonly caused by Candida albicans; however, an increase in the prevalence of non-albicans species has been observed during last decades. This study aimed at molecular identification of Candida species isolated from candiduria in hospitalized patients.
Methods: This cross-sectional study was conducted on 530 hospitalized patients in two hospitals in the Mazandaran Province, Iran. Midstream urine specimens were collected and then cultured on CHROMagar Candida medium. Molecular identification of common Candida species was carried out using the polymerase chain reaction-restriction fragment length polymorphism method after enzymatic digestion with MspI. C. albicans and Candida parapsilosis species complexes were identified by amplification of the HWP1 and intein-containing vacuolar ATPase precursor genes, respectively.
Results: The frequency of candiduria was estimated at 14% among hospitalized patients. Of 74 samples positive for candiduria, 65 (87.8%) were isolated from females. The most common predisposing factor to candiduria was diabetes (n=36; 48.6%). The most frequent isolates were C. albicans complex (n=44; 59.4%), followed by Candida glabrata (n= 16; 21.6%), Candida tropicalis (n= 10; 13.5%), Candida Krusei (n= 3; 4%) and C. parapsilosis (n= 1; 1.3%).
Conclusion: Based on the results, the conventional and molecular methods produced similar results for identification of Candida species. However, accurate identification of Candida spp. requires the use of molecular techniques such as PCR-RFLP, HWP1, and intein-containing vacuolar ATPase precursor genes. Nevertheless, chromogenic methods such as CHROMagar Candida can be used for diagnosis of Candida spp. in laboratories with limited resources.
Sajad Jafari, Mohammad Fathi, Masoud Rahmati,
Volume 17, Issue 3 (5-2023)
Abstract
Background and objectives: Endurance exercise causes fatigue due to mitochondrial dysfunction and oxidative stress. The present study was designed to investigate the effects of taurine supplementation on lipids peroxidation and antioxidant activity during endurance activities.
Methods: Twenty-four male volunteers aged 27 ± 1.8 years and weighting 74.9 ± 5.9 kg were randomly divided into three groups: taurine supplementation (n=8), placebo (n=8), and control (n=8). The subjects completed a 28-day endurance training protocol. Biochemical parameters such as superoxide dismutase (SOD), glutathione peroxidase (GPX) activities, as well as malondialdehyde (MDA) concentrations (8 hours before the first session and 8 hours after the last session) and maximum rate of oxygen consumption, were measured to evaluate the antioxidant, lipid peroxidation, and VO2 max status respectively. Finally, data were analyzed by SPSS software at a significance level of <0.05.
Results: Taurine supplementation significantly increased SOD (p=0.001) and GPX (p=0.001) but significantly decreased MDA (p=0.001). However, it had no significant effect on the VO2 max.
Conclusion: The results of the present study indicate that taurine has antioxidant effects against endurance exercise-induced oxidant stress and lipid peroxidation.
