Thu, Jul 24, 2025
Saeid Anvari1 
, Setare Kheyrandish2 , Fatemeh Sotudeh3 , Hossein Mirpour Hasankiadeh4 , Korosh Khanaki3 , Esmaeil Shahabi Satlsar5
, Setare Kheyrandish2 , Fatemeh Sotudeh3 , Hossein Mirpour Hasankiadeh4 , Korosh Khanaki3 , Esmaeil Shahabi Satlsar5
1- Regenerative Medicine, Organ Procurement and Transplantation Multi-diciplinary Center, Razi Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
2- Hematology and Blood Banking Department, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Langeroud School of Paramedical Sciences, Guilan University of Medical Sciences, Rasht, Iran
4- Department of Internal Medicine, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
5- Langeroud School of Paramedical Sciences, Guilan University of Medical Sciences, Rasht, Iran ,esmaeilshahabi@yahoo.com
2- Hematology and Blood Banking Department, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Langeroud School of Paramedical Sciences, Guilan University of Medical Sciences, Rasht, Iran
4- Department of Internal Medicine, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
5- Langeroud School of Paramedical Sciences, Guilan University of Medical Sciences, Rasht, Iran ,
Abstract: (1217 Views)
Chronic basophilic leukemia (CBL) is a rare disorder and according to the published data, few cases have been reported as primary CBL. Morphologic findings in CBL mostly mimic chronic eosinophilic leukemia and basophils have unusual and dysplastic morphology. A 47-year-old patient was referred to the hospital with gastrointestinal symptoms and bloating. Complete blood count (CBC) showed leukocytosis and marked eosinophilia. After evaluating the peripheral blood smear (PBS), abnormal leukocytes with hypersegmented nuclei along with an increase in eosinophils were observed. For further investigation, the patient was referred to the hematology clinic and underwent bone marrow aspiration and biopsy. After the morphological examination, flow cytometry was performed on the aspiration sample to accurately diagnose the disease. Flow cytometric findings were in favor of CBL. Since morphological findings are unreliable for basophil detection and definite diagnosis, flow cytometry is a reliable method for the precise detection of basophils, especially in CBL.
Keywords: Basophils, Flow Cytometry, Myeloproliferative Disorders, Chronic Basophilic Leukemia, Chronic Eosinophilic Leukemia
Research Article: Case Report |
Subject:
Laboratory hematology
Received: 2023/03/12 | Accepted: 2023/07/17
Received: 2023/03/12 | Accepted: 2023/07/17
References
1. Khoury JD, Solary E, Alba O, Akkari Y, Alaggio R, Apperley JF, et al. The 5th edition of the World Health Organization Classification of Haematolymphoid Tumores: Myeloid and Histiocytic/Dendritic Neoplasm. Leukemia. 2022;36(7):1703-19. [View at Publisher] [DOI] [PMID] [Google Scholar]
2. Cehreli C. Diagnostic problems in chronic basophilic leukemia. Turk J Hematol. 2018;35(4):283-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Valent P, Sotlar K, Blatt K, Hartmann K, Reiter A, Sadovnik I, et al. Proposed diagnostic criteria and classification of basophilic leukemia and related disorders. Leukemia. 2017;31(4):788-97. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Pardanani AD, Morice WG, Hoyer JD, Tefferi A. Chronic Basophilic Leukemia: a distinct clinic-pathologic entity? Eur J Hematol. 2003;71(1):18-22. [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Bain BJ, Heller M. Dysplastic basophils in the accelerated phase of chronic myelogenous leukemia. Am J Hematol. 2011;86(11):949. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Tang G, Woods LJ, Wang SA, Brettler D, Anderson M, Miron PM, et al. Chronic Basophilic Leukemia: a rare form of chronic myeloproliferative neoplasm. Hum Pathol. 2009;40(8):1194-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. Valent P, Horny HP, Arock M. The underestimated role of basophils in Ph+ chronic myeloid leukemia. Eur J Clin Invest. 2018;48(10):e13000. [View at Publisher] [DOI] [PMID] [Google Scholar]
8. Stacchini A, Demurtas A, Godio L. Flow cytometric detection of of degranulated basophils in chronic myeloid leukemia in accelerated phase. Cytometry B Clin Cytom. 2011;80B(2):122-4. [View at Publisher] [DOI] [PMID] [Google Scholar]
9. Han X, Jorgensen JL, Brahmandam A, Schlette E, Huh YO, Shi Y, et al. Immunophenotypic study of basophils by multiparameter flow cytometry. Arch Pathol Lab Med. 2008;132(5):813-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
10. Chehreli C, Ates H, Chehreli R, Sercan Z, Demirkan F. New paraneoplastic syndrome in chronic basophilic leukemia. Int J Hematol. 2013;97(4):498-504. [View at Publisher] [DOI] [PMID] [Google Scholar]
11. Cehreli C, Alacacioglu I, Piskin O, Ates A, Cehreli R, Calibasi G, et al. Mast cell leukemia associated with undefined morphology and chronic basophilic leukemia. BMC Hematol. 2014;14:17. [View at Publisher] [DOI] [PMID] [Google Scholar]
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.