Sat, May 31, 2025
Zahra Eslami1 
, Shayan Marhamaty2 , Mehdi Jafari1 , Mohadese Khorasani3 , Mehdi Sheikh Arabi4 , Hamidreza Joshaghani5
, Shayan Marhamaty2 , Mehdi Jafari1 , Mohadese Khorasani3 , Mehdi Sheikh Arabi4 , Hamidreza Joshaghani5
1- Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Golestan province, Iran
2- student of clincal biochemistry, Hamadan University of Medical Sciences, Hamadan, Hamadan province, Iran
3- Ph.D. student of analytical chemistryLaboratory Assistant at Golestan University of Medical Sciences
4- Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran
5- Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran ,joshaghani@goums.ac.ir
2- student of clincal biochemistry, Hamadan University of Medical Sciences, Hamadan, Hamadan province, Iran
3- Ph.D. student of analytical chemistryLaboratory Assistant at Golestan University of Medical Sciences
4- Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran
5- Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran ,
Abstract: (466 Views)
background:
Bivalent minerals have an important role as cofactors which play vital roles in various metabolic pathways in the body. Zinc (Zn) has catalytic, structural, and regulatory roles. Severe Zn deficiency may cause the abnormal synthesis of nucleic acids, and proteins, impaired cellular growth, excessive cell death, and excess lipid peroxidation of the cellular membrane that is associated with shortening the RBC lifespan.
Purpose:
The aim of this study was to examine the associations between Zn status and the erythrocyte indices.
Methods and materials:
A number of 563 individuals (72.8% female) were included in this study. The level of serum Zn was measured by photometric method and blood index values were measured by using a cell counter.
Results:
The average serum Zn level is 102.8± 17.6 mg/dl. Serum Zn level is directly related with RBC (R=0.119, PV=0.005) and MCHC (R=0.086, PV=0.041) but it is inversely related with MCV (R=-0.097, PV=0.021). These results also determined that serum Zn level, as well as the levels of RBC, Hb, HCT, and MCHC, were significantly higher in men (Sig<0.01) but the level of MCV among women was higher (Sig<0.01). Moreover, in individuals with <30 serum Zn level, MCHC (Sig<0.01), and RBC (Sig<0.05) were higher whereas Hb (Sig<0.05), HCT, MCV, and MCH (Sig<0.01) were higher at >30.
Conclusion:
According to the positive relationship between Zn level and RBC, Zn deficiency affects the number of RBCs.
Bivalent minerals have an important role as cofactors which play vital roles in various metabolic pathways in the body. Zinc (Zn) has catalytic, structural, and regulatory roles. Severe Zn deficiency may cause the abnormal synthesis of nucleic acids, and proteins, impaired cellular growth, excessive cell death, and excess lipid peroxidation of the cellular membrane that is associated with shortening the RBC lifespan.
Purpose:
The aim of this study was to examine the associations between Zn status and the erythrocyte indices.
Methods and materials:
A number of 563 individuals (72.8% female) were included in this study. The level of serum Zn was measured by photometric method and blood index values were measured by using a cell counter.
Results:
The average serum Zn level is 102.8± 17.6 mg/dl. Serum Zn level is directly related with RBC (R=0.119, PV=0.005) and MCHC (R=0.086, PV=0.041) but it is inversely related with MCV (R=-0.097, PV=0.021). These results also determined that serum Zn level, as well as the levels of RBC, Hb, HCT, and MCHC, were significantly higher in men (Sig<0.01) but the level of MCV among women was higher (Sig<0.01). Moreover, in individuals with <30 serum Zn level, MCHC (Sig<0.01), and RBC (Sig<0.05) were higher whereas Hb (Sig<0.05), HCT, MCV, and MCH (Sig<0.01) were higher at >30.
Conclusion:
According to the positive relationship between Zn level and RBC, Zn deficiency affects the number of RBCs.
Research Article: Original Paper |
Subject:
Laboratory hematology
Received: 2023/07/25 | Accepted: 2023/10/2
Received: 2023/07/25 | Accepted: 2023/10/2
References
1. King JC, Brown KH, Gibson RS, Krebs NF, Lowe NM, Siekmann JH, et al. Biomarkers of Nutrition for Development (BOND)-Zinc Review. J Nutr. 2015; 146(4): 858s-85s. [View at Publisher] [DOI] [PMID] [Google Scholar]
2. Önal S, Nazıroğlu M, Çolak M, Bulut V, Flores-Arce MF. Effects of different medical treatments on serum copper, selenium and zinc levels in patients with rheumatoid arthritis. Biol Trace Elem Res. 2011;142(3):447-55. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Wood RJ. Assessment of Marginal Zinc Status in Humans. The Journal of nutrition. 2000; 130(5): 1350S-4S. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Gupta S, Brazier A, Lowe N. Zinc deficiency in low‐and middle‐income countries: prevalence and approaches for mitigation. Journal of Human Nutrition and Dietetics. 2020; 33(5): 624-43. [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Bartakke S, Bavdekar SB, Kondurkar P, Muranjan MN, Manglani MV, Sharma R. Effect of deferiprone on urinary zinc excretion in multiply transfused children with thalassemia major. Indian Pediatr. 2005; 42(2): 150-4. [View at Publisher] [PMID] [Google Scholar]
6. Erdoğan E, Canatan D, Ormeci AR, Vural H, Aylak F. The effects of chelators on zinc levels in patients with thalassemia major. J Trace Elem Med Biol. 2013; 27(2): 109-11. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. Kelkitli E, Ozturk N, Aslan NA, Kilic-Baygutalp N, Bayraktutan Z, Kurt N, et al. Serum zinc levels in patients with iron deficiency anemia and its association with symptoms of iron deficiency anemia. Ann Hematol. 2016; 95(5): 751-6. [View at Publisher] [DOI] [PMID] [Google Scholar]
8. Ozturk Z, Genc GE, Gumuslu S. Minerals in thalassaemia major patients: an overview. Journal of Trace Elements in Medicine and Biology. 2017; 41: 1-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
9. Al-Samarrai AH, Adaay MH, Al-Tikriti KA, Al-Anzy MM. Evaluation of some essential element levels in thalassemia major patients in Mosul district, Iraq. Saudi Med J. 2008; 29(1): 94-7. [View at Publisher] [PMID] [Google Scholar]
10. Bonfils L, Ellervik C, Friedrich N, Linneberg A, Sandholt CH, Jørgensen ME, et al. Fasting serum levels of ferritin are associated with impaired pancreatic beta cell function and decreased insulin sensitivity: a population-based study. Diabetologia. 2015; 58(3): 523-33. [View at Publisher] [DOI] [PMID] [Google Scholar]
11. Fung EB, Gildengorin G, Talwar S, Hagar L, Lal A. Zinc status affects glucose homeostasis and insulin secretion in patients with thalassemia. Nutrients. 2015; 7(6): 4296-307. [View at Publisher] [DOI] [PMID] [Google Scholar]
12. Rehman S-u. Effects of zinc, copper, and lead toxicity on α-aminolevulinic acid dehydratase activity. Bulletin of environmental contamination and toxicology. 1984; 33: 92-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
13. Osawa M, Yamaguchi T, Nakamura Y, Kaneko S, Onodera M, Sawada K, et al. Erythroid expansion mediated by the Gfi-1B zinc finger protein: role in normal hematopoiesis. Blood. 2002; 100(8): 2769-77. [View at Publisher] [DOI] [PMID] [Google Scholar]
14. Aoki C, Imai K, Owaki T, Kobayashi-Nakano T, Ushida T, Iitani Y, et al. The Possible Effects of Zinc Supplementation on Postpartum Depression and Anemia. Medicina (Kaunas). 2022;58(6). [View at Publisher] [DOI] [PMID] [Google Scholar]
15. Yanagisawa H, Miyakoshi Y, Kobayashi K, Sakae K, Kawasaki I, Suzuki Y, et al. Long-term intake of a high zinc diet causes iron deficiency anemia accompanied by reticulocytosis and extra-medullary erythropoiesis. Toxicology letters. 2009;191(1):15-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
16. Akodu SO, Njokanma OF, AdeoluKehinde O. Erythrocyte indices in pre-school Nigerian children with sickle cell anaemia in steady state. International journal of hematology-oncology and stem cell research. 2015; 9(1): b5. [View at Publisher] [PMID] [Google Scholar]
17. Kim S, Lee K, Kim I, Jung S, Kim M-J. Red cell distribution width and early mortality in elderly patients with severe sepsis and septic shock. Clinical and experimental emergency medicine. 2015; 2(3): 155. [View at Publisher] [DOI] [PMID] [Google Scholar]
18. Angelova MG, Petkova-Marinova TV, Pogorielov MV, Loboda AN, Nedkova-Kolarova VN, Bozhinova AN. Trace Element Status (Iron, Zinc, Copper, Chromium, Cobalt, and Nickel) in Iron-Deficiency Anaemia of Children under 3 Years. Anemia. 2014; 2014: 718089. [View at Publisher] [DOI] [PMID] [Google Scholar]
19. Ece A, Uyanik BS, Işcan A, Ertan P, Yiğitoğlu MR. Increased serum copper and decreased serum zinc levels in children with iron deficiency anemia. Biol Trace Elem Res. 1997; 59(1-3): 31-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
20. Chang H, Xu A, Chen Z, Zhang Y, Tian F, Li T. Long-term effects of a combination of D-penicillamine and zinc salts in the treatment of Wilson's disease in children. Exp Ther Med. 2013; 5(4): 1129-32. [View at Publisher] [DOI] [PMID] [Google Scholar]
21. Brewer GJ, Hill GM, Prasad AS, Cossack ZT, Rabbani P. Oral zinc therapy for Wilson's disease. Ann Intern Med. 1983; 99(3): 314-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
22. Harada M, Miyagawa K, Honma Y, Hiura M, Shibata M, Matsuhashi T, et al. Excess copper chelating therapy for Wilson disease induces anemia and liver dysfunction. Intern Med. 2011; 50(14): 1461-4. [View at Publisher] [DOI] [PMID] [Google Scholar]
23. Cai S, Gong JY, Yang J, Wang JS. Anemia following zinc treatment for Wilson's disease: a case report and literature review. BMC Gastroenterol. 2019;19(1):120. [View at Publisher] [DOI] [PMID] [Google Scholar]
24. Pilch SM, Senti FR. Analysis of zinc data from the second National Health and Nutrition Examination Survey (NHANES II). J Nutr. 1985; 115(11):1393-7. [View at Publisher] [DOI] [PMID] [Google Scholar]
25. Cavan KR, Gibson RS, Grazioso CF, Isalgue AM, Ruz M, Solomons NW. Growth and body composition of periurban Guatemalan children in relation to zinc status: a longitudinal zinc intervention trial. The American journal of clinical nutrition. 1993; 57(3): 344-52. [View at Publisher] [DOI] [PMID] [Google Scholar]
26. Smit Vanderkooy P, Gibson R. Food consumption patterns of Canadian preschool children in relation to zinc and growth status. The American journal of clinical nutrition. 1987; 45(3): 609-16. [View at Publisher] [DOI] [PMID] [Google Scholar]
27. Van Wouwe JP. Clinical and laboratory assessment of zinc deficiency in Dutch children. Biological trace element research. 1995; 49(2): 211-25. [View at Publisher] [DOI] [PMID] [Google Scholar]
28. Walravens PA, Krebs NF, Hambidge KM. Linear growth of low income preschool children receiving a zinc supplement. The American journal of clinical nutrition. 1983; 38(2): 195-201. [View at Publisher] [DOI] [PMID] [Google Scholar]
29. Shils ME, Olson JA, Shike M. Modern nutrition in health and disease. Lea & Febiger, Philadelphia. 1994. [View at Publisher] [Google Scholar]
30. Haase H, Mocchegiani E, Rink L. Correlation between zinc status and immune function in the elderly. Biogerontology. 2006;7(5-6):421-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
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